1 OD at 230 nm. For more sample throughput, both readers can be easily integrated into full robotic Microplate Readers. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. Learn more. 5. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. ZERO BIAS. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. Learn more 0200-7008 6- to 1536-well plates. ODs were measured in 96 well plates with a plate reader (Molecular Devices, SpectraMax ABS Plus) with two technical replicates of each of. Workflows of the MTT Cell Proliferation and Cell Cytotoxicity Assay. 96-well and 384-well plates; Wavelength(s): 405, 450, 490, 560 and 600nm (Abs) Specifications/Features: Shaking, Temperature control, Injector. both cuvette-based and microplate reader applications SpectraMax Mini Multi-Mode Microplate Reader. Make sure to Data 96孔微孔板的数据对比. for 96- and 384-well microplates • Detect dim samples with unsurpassed sensitivity • Avoid saturation issues with nine orders of dynamic range • Enable robust performance with autorinse injectors • Validation and compliance tools SpectraMax® L Microplate . 00. adapter plate in drawer of Plate Reader with well A1 in position A1 of the drawer. 125 pM * Specifications listed are for europium 96-well: 0. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. #3676). New SpectraMax iD5 Multi-mode Microplate Reader. Both COC microplates, the µCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. 0200-6262. Plate Reader with Microplate Adapter, Molecular Devices SpectraMax M series Multi-mode 6. We would like to show you a description here but the site won’t allow us. Both readers were heated to 37ºC prior to setting up the assay. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. The validation kit included individual tubes of diluted fluorophores:. 0 500 1000 1500 2000 2500 3000 3500 time (sec. Standard SBS 96 and 384 well plates (128. If the best wavelengths are unknown, the Gemini EM reader will find. Brand: MOLECULAR DEVICES. #ABS PLUS) Methods. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader. Published Dec 16, 2020 | Updated Dec 16, 2020. 14: Average %CV. 76 L x 85. , Sunnyvale, CA 94089. Note: SoftMax Pro Software must be installed on a computer connected to the instrument. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. HeLa cells were plated at 15,000 cells per well in 100 µL of medium in a black, clearbottom microplate. ValitaTiter 96-well (Pack of 20). Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. ; Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows:. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories. the appropriate well of the microplate. 05. Our microplate. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. This document outlines references to 21 CFR Parts 58, 211 and 820 and EudraLex Annex 15 to assess the implementation of Molecular Devices microplate readers in regulated environments. Materials. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. Built in near field communication (NFC). The reader includes multiple licenses to the SoftMax® Pro software for endpoint and kinetic analysis of microplate. The EMax Plus Microplate Reader measures 96-well plates and comes with eight standard filter modes to cover the entire visible range, for applications such as protein quantification, cell viability, cell proliferation and ELISA. It also integrates with the. We would like to show you a description here but the site won’t allow us. The SpectraMax Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Cells were washed three times with calcium wash buffer using an AquaMax 4000 Microplate Washer with 96- or 384-well cell wash head. Molecular Devices 推出了FlexStation® 3 多功能读板机系统。FlexStation 3 把 SpectraMax M5e 和 8 道 / 16 道的移液 系统整合在一起成为体积小巧功能强大的 读板机系统。该整合系统不但为用户提供 了多功能检测平台，还增加了液体处理的 通量和提升了检测生化和细胞动. ***Figure 1. flexibility of monochromator-based systems. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. The direct transfer capability reduces reagent consumption and allows for more assay conditions to be explored in a single microplate, making the system more amenable to agonist and antagonist assay formats. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. (Figure 1). Other technologies such as imaging. 2 2601A ValitaTiter. Injectors Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. 003 fmol 0. Jon Cuccui, Brendan W. 384 Awareness Technology ChroMate BioTek Synergy Mx. Documentation:1. The VersaMax and SpectraMax 190 read 96-well microplates. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. This reader also makes use of a unique temperature-independent methodology for path length correction. Published Dec 16, 2020 | Updated Dec 16, 2020. MultiWash+ Microplate Washer, A 96- & 384-well Automated Washer| Molecular Devices Author: Molecular Devices, LLCA polyclonal antibody specific to mouse/rat IL-22 was pre-coated onto a 96-well microplate. 2. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. With various on-board features and capabilities, the ThermoMax is. 1. Samples were run in triplicate. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. 05. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. The system comes with a single plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . The clear UltraVision FC 96-well microplate (FC_UltraVision (clear, 96)) showed the best performance of the tested microplates, with absorbance of 0. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ Microplate Washer. The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the assay. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well3. An industry-renowned instrument for monitoring GPCRs and ion channels. # 0310-5218T). Learn how Microplate Washer configured for both 96- and 384-well plates that help scientists by providing transformational workflow into practice, to automate a tedious process, to do more with less. buffers for (2) 96-well microplates R8088 CatchPoint cAMP 96-well Fluorescent Assay Kit (Bulk) ELISA-based assay for the detection and quantification of cAMP * Contains microplates, reagents, and buffers for (10) 96-well microplates R8089 SpectraMax® i3/i3x Multi-Mode Microplate Reader SpectraMax® Paradigm® FlexStation® 3 Multi-Mode. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. This report demonstrates that the SPECTRAmax GEMINI-XS microplate spectrofluorometer, with its dual scanning monochromator and patented optical sensitivity, can be used to detect a relatively low number of cells expressing GFP. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate:The EMax® Endpoint ELISA Microplate Reader from Molecular Devices, one of the pioneers in microplate detection, offers a ruggedly simple approach to dedicated endpoint assays. Reader. microplate reader (Danaher Inc) Danaher Inc is a verified supplier Danaher Inc manufactures this product . Microplates . Microplates, also known as microtiter plates, are rectangular multi-well plates used in a variety of assays from ELISA to PCR. In combination with the SpectraMax Mini Multi-Mode Microplate Reader, it enables increased throughput and detection of DNA as low as 2 ng/μL using just a few microliters of sample. Add 150 µL reagent to each well. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96- or 384-well head. Bulk Kit (10-plate size): Molecular Devices P/N R8341; HeLa cells (ATCC P/N CCL-2) Staurosporine (Sigma P/N S5921) 96-well black, clear-bottom microplates (Corning P/N 3904) SpectraMax fluorescence microplate reader; Methods. Flexible for 6-well to 384-well microplates and cuvettes. is required for optimum performance with standard 96-well and 384-well format microplates for all top-read applications. Measuring Time: Reading Times (Flying Mode 1 flash): 9 sec (96), 16 sec (384) Wavelength Range:Both the MTT and Cell Cytotoxicity assays were read on the SpectraMax® ABS Plus Microplate Reader, which can read a 96-well microplate in less than 10 seconds. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. Related Products: Low Binding Plates. Next generation SpectraMax® absorbance microplate readers. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read:. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. fluorescence microplate reader from Molecular Devices. The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. It also integrates with the powerful SoftMax Pro Software for. The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise. Step 2: Launch SoftMax Pro Software. The EMax® Plus Microplate Reader is designed to be a versatile and robust microplate reader for research laboratories. White 96-well plate The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. This is equivalent to having 511 built-in filters,Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. to each well. Read the microplate Step 1: Place the microplate in the reader. 04 sec. ScanLater Western Blot Detection System. is required for optimum performance with standard 96-well and 384-well format microplates for all top-read applications. A multitude of modalities. Formats available are strip washers, full plate washers and combination washer-dispensers. Both readers were heated to 37ºC prior to setting up . The microplate reader then generates data following a preconfigured protocol that performs the necessary calculations on the data, delivering consistent results. Page 154: Ordering Parts And Accessories. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. 2 mm x 86 mm) Height: between 13. # 0310-5217T) 384-well cell wash head (Molecular Devices Cat. SpectraMax® M3/M4/M5/M5e, GeminiTM EM/XPS, FlexStation® 3. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. ©2017 Molecular Devices, LLC . Software Validation. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. 020 fmol 0. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. Optimized optical path - The reader’s unique quartz fiber optic system minimizes stray light for precise readings across a microplate. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. BMG LABTECH. When the user applies a set temperature of 37°C, the top is heated to 37. U-bottom: 30-40 µl. After 5, 7, 11, and 13 days. Microplate readers use a vertical light path so the distance of the. This unit offers a wavelength range of 340 nm to 850 nm and a bandwidth of 5 nm. #VAL004 or VAL014) 96-well assay plate • Human IgG for use as standard (e. Fluorescence Microplate Readers. HeLa cells were plated at 20,000 cells per well in 100 µL per well in a black, clear-bottom microplate. 3 OD at 200 nm. ValitaTiter Assays Setup Guide 2601A October 2023. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. 1 sec. The top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. This reader's dual monochromators for. Its software interface is easy to learn and use, and lets you control the instrument from almost any device –. The SpectraMax Plus 384 Microplate Reader for absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. molecular devices gmbh tel. The kinetic top-read mode of a SpectraMax M3 microplate reader (Molecular Devices, CA, USA). This reader provides visible. book Page 9 Wednesday, July 7, 2010 2:09 PM. Reconstitution of reagents. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. AquaMax 4000 Microplate Washer (Molecular Devices Cat. 5 mm. Programmable liquid handling allows for perfectly timed, automated biochemical and cell-based assays. SpectraMax M5eMulti-Mode Microplate Reader (Molecular Devices cat. Published Dec 16, 2020 | Updated Dec 06, 2021. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. 0 H x 60. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. Gemini EM and SpectraMax M2 Microplate Readers By Yan Zhang, Molecular Devices Corporation, 1311 Orleans Dr. This page contains the. University of Reading Simon Lydford: Molecular Devices (UK) Ltd. SpectraMax Mini Multi-Mode Microplate Reader Afordable multi-mode microplate reader with best-in-class data analysis software Perform your favorite applications including. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. #VAL004 or VAL014) 96-well assay plate • Human IgG for use as standard (e. The system comes with a single plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and Bottom read. Molecular Devices SpectraMax 384 Plus Microplate Reader. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. The absorbance at 412 nm was measured by a SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA) at 30 °C. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofMulti-mode microplate readers with fluorescence detection. Use black microplates. 96-well solid bottom, clear polystyrene microplates (Greiner cat. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. absorbance, fluorescence, and luminescence with the added functionality of. 300 µL of Detergent Compatible Bradford reagent was added to each well. 00 Add to cart; Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. 2 OD at 200 nm; this decreased to 0. 03 pM 384-well: 0. #VAL003 or VAL013) or ValitaTiter Plus (Molecular Devices cat. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. 1. Setup Guide. VersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. High. section, select plate section titled. 3. and reliable microplate stacking solution for Molecular Devices microplate readers and washers. Published Dec 16, 2020 | Updated Dec 16, 2020. g. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Application Area: Spectrophotometer for molecular biology. A control well was set up to verify. 96-well plates: V-bottom: 20-30 µl U-bottom: 30-40 µl flat-bottom: 50-60 µl 384-well plates: approximately 20 µl Recommended ArticlesA method is described for the routine determination of the rate of colorimetric enzyme reactions using a 96-well microtiter plate reader commonly used in. 3. Other Molecular Devices readers with absorbance detection mode, e. Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. 96-well microplate; Immunoassay analyzer;VersaMax price. gemini em reader, molecular devices stakmax microplate stacker, softmax pro data analysis, spectrofluorometer, monochromator microplate reader, cell migration assays, cell viability assays, gfp, picogreen dna. /well integration in 96-well luminescence mode and 0. #N207) LAL Reagent Water (Lonza cat. Retrieving. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. (12 and 96 well) all: FMax: all: LMax: all: Macro 96 Well Cell Harvester: all: Micro 96 Well Cell Harvester: all: Mini 96 Well Cell HarvesterSpectraMax L Microplate Reader A flexible, sensitive luminometer for 96- and 384-well microplates Key Features • Unsurpassed sensitivity • Low background and cross-talk • Multi-channel configurations for a 6-fold. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. Plate Formats: 6 to 384-well microplates. DNA concentration (ng/mL) Average RFU. 4% in white 96- and 384-well microplates Sensitivity (ATP-Glow) Optimized 96 wells 3 pM 384 wells 6 pM * With 6 flashes in absorbance and 3 flashes in fluorescence mode and 0. For FlexStation I/II/3 plate readers, what are the dead-volumes for the compound plate? The FlexStation plate readers assume a 1 mm pipette height from the bottom of the well when aspirating from a compound source plate. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. In addition, the iD5 reader measures TRF and FP and. robotics-automation-compatible. ) 20,000 10,000 30,000 40,000 50,000 60,000 70,000 80,000 0. Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. Instrument settings can be saved as a protocol file and used repeatedly for reading differentMolecular Devices SpectraMax ® Paradigm ® Multi-Mode Microplate Detection Platform is a user upgradeable microplate reader that allows for real-time system configuration by the user in less than two minutes. 96- or 384-well microplate compatibility. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. #T-0254) • Lysozyme (Sigma cat. Multimode Readers Comparison Chart. 96-well: 0. research hardware on the International Space Station, providing researchers the ability to conduct microplate reader experiments in microgravity for the first time. Related Products: Low Binding Plates. Industry standard, 96-well plates are. A 1:10 serial dilution of ATP was prepared in medium. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. SoftMax Pro Software’s SpectralThe Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. The onboard luminescence detection mode may be used, or, for higher sensitivity, the reader can be equipped with a NanoBRET detection cartridge that offers a 4-fold improvement in limit of quantitation. Multimode Reader Comparison Chart. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. Simple, economical . SpectraMax 190 and VersaMax readers can accommodate standard 96-well and strip-well microplates. Abstract and Figures. Temperature control. Expt#3 . Description. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. we demonstrate how Molecular Devices microplate readers with luminescence detection mode provide superior sensitivity and ease of use for reliable mycoplasma detection using MycoAlert. With optimized reagents, option to operate the. 96-well microplate at 200 µL/well. The Perkin Elmer Wallac 1420. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Page 20: Plate Adapter SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the. 4D-Nucleofector 96-well Shuttle: RATE CONTROLLED FREEZING: Grant: Asymptote EF600M Freezer:. SpectraMax 250 User. It also allows for the elimination of standard curves. The SpectraMax ABS Plus Microplate Reader can run both cuvette-based and microplate based reader applications on the same instrument. The SpectraMax® iD5 Multi-mode Microplate Readers measure absorbance, fluorescence, and luminescence. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Read absorbance on the SpectraMax microplate reader at the wavelength to be used for your assay, and average the resulting OD values for all wells to obtain the Plate. SpectraMax ABS Plus reader utilizes Molecular Devices PathCheck Sensor- the only patented. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. No additional manuals are available. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96- or 384-well head. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. 03 pM 384-well: 0. 9. Page 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. Place a lid on the microplate and put the plate in the drawer of the reader and mix for approximately 5 seconds. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. In May 2011, Molecular Devices SpectraMax M5e reader, chosen for its durability, became the first microplate reader . The device was able to achieve 3σ limits of detection of ca. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Molecular Devices microplate readers have the most variety of tools available for users to meet their diverse hardware and software validation needs. SpectraMax Microplate Readers. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Less background noise gives a better limit of detection and so a relative increase in usable signal. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate. 2 OD at 200 nm. We would like to show you a description here but the site won’t allow us. 1. Optical absorbance and fluorescence were measured on a SpectraMax M5 Multi-Mode microplate reader (Molecular Devices, San Jose, CA). For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. In . We demonstrate that miniaturizing the assay in 96-well half area microplates, and using the flexible liquid transfer features of the FlexStation ® 3 Multi-Mode Microplate Reader allows rapid and reliable measurement of EC 50 values for agonists and IC 50 values for antagonists. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. #N207) LAL Reagent Water (Lonza cat. No filter change with dual monochromator optics. The StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. Deep well blocks available in 24, 96 and 384-well formatsFlexStation® 3 Multi-Mode Microplate Readers . The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M4 Microplate Reader transforms each well in a microplate to a fixed. The system provides automation for up to 50 microplates for easy walkaway automation. Four wash/rinse bottles are included and configurable for both 96- and 384. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. CHO-M1 cells were plated at a density of 50,000 cells per well (96-well) or 12,500 cells per well (384-well) the day prior to assay. 012 fmol 0. for ValitaTiter Plus standards read on Molecular Devices readers. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. CloneMatrix. Molecular. This report demonstrates that the SPECTRAmax GEMINI-XS microplate spectrofluorometer, with its dual scanning monochromator and patented optical sensitivity, can be used to detect a relatively low number of cells expressing GFP. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec. Cross-talk < 0. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. It includes both shaking and incubation functions, and is compatible with 96- well microplates. Place the microplate in the drawer. Outer wells are aligned for proper viewing with FLIPR. 6. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. The FilterMax F5 can read 6, 12, 24, 48, 96, 384, 1536-well. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Monochromator, tunable in 1-nm increments. acquisition and SoftMax Pro SoftwareMiniMax Imaging Edition. Instrument settings can beAbsorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. LVF Monochromator™ : Excitation and Emission. Molecular Devices gmbH Tel. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. Never order or replace filters to optimize an assay again!microplate readers from Molecular Devices. SpectraMax Glo Steady-Luc Reporter Assay Kit, Molecular Devices (Explorer kit #R8352 or Bulk kit #R8353) Competitor kit: Steady-Glo Luciferase Assay System, Promega (Cat# E2510) SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903) Purified luciferase, Promega (Cat# E1701) The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. The reduced cost of the device represents the major technical contribution of this manuscript, as costs were reduced 7 fold from previous reports. Molecular devices The VersaMax Absorbance Microplate Reader costs between $5,500 and $6,600. < 0. 39. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Making it possible to perform kinetic analysis of solid. 96-well: 0. 96-well microplate at 200 µL/well. Running both standard spectrophotometer and microplate.